Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 250G
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 250G
Alkali Scientific CellPro™ Tris-Glycine SDS Running Buffer, 10X, pH 8.3, 100mL
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(t-Bu) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 100 MG
NODAGA-tris(t-Bu ester) is a tri-tert-butyl ester-protected NODAGA chelator intended for conjugation to biomolecules and subsequent radiolabeling for positron emission tomography (PET) studies. It provides a protected, bifunctional scaffold that facilitates attachment to peptides, proteins, and antibodies and enables stable chelation of radiometals after deprotection.
- Protected NODAGA chelator for conjugation to biomolecules.
- Suitable for labeling peptides, proteins, and antibodies.
- Designed for radiolabeling workflows for PET imaging.
- High purity (99.8%) with RP-HPLC documentation available.
- Available as solid and as DMSO solutions for convenience.
- Molecular formula C27H49N3O8; molecular weight 543.69 g/mol.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 100g
BIS-TRIS | Purity: ≥99.0% (NT) | MW:209.24 | 6976-37-0 | MFCD00002853 | 100g
LAB BIOREAGENTS LowBinding IV Buffer 250ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Abcam Salsolinol hydrochloride, Dopamine derivative, 5G
MW 215.68 g/mol, Purity >99%. Dopamine (ab120565) derivative. Putative endogenous opioid system-activating factor. Antagonizes L-DOPA-mediated hyperactivity. Shows central effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement